JIST

Abstract

Suicidal and accidental poisoning with drug overdose isone of the common causes of morbidity and mortality in India. Considering such fact, quantification of drug becomes imperative to distinguish narrow margin of therapeutic dose and toxic dose. Acetaminophen (Paracetamol) is extensively used as antipyretic and analgesic drug. Generally it is used in headaches, slight pains and fever. Overdose of Paracetamol causes hepatotoxicity. It can be fatal in large dose (>10g).It can potentiate the harmful effect of other drugs if taken in combination. A quick, simple and precise method is developed for quantitative estimation of acetaminophen using RP-HPLC in biological samples viz. Blood, Vitreous Humor and CSF. The separation was done using RPC-18 HPLC column and Methanol: water as mobile phase. On the basis of ICH guidelines validation parameters such as Linearity, Accuracy, Precision, Recovery, Limit of Quantification and Limit of Detection were done.